Microfluidics and multielectrode array-compatible organotypic slice culture method
نویسندگان
چکیده
منابع مشابه
Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain ...
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AIMS Cardiovascular research requires complex and functionally intact experimental models. Due to major differences in the cellular and subcellular composition of the myocardium between species, the use of human heart tissue is highly desirable. To enhance the experimental use of the human myocardium, we established methods for the preparation of vital tissue slices from the adult ventricular m...
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Early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as socalled organotypic brain slice cultures. The present study describes a simple method for stationary long-term organotypic culture for the hypothalamic suprachiasmatic nucleus (SCN). Hypothalamic brain slices from ...
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the exact developmental origin of microglia is still under debate. in the present study we investigated which heamatopoietic tissues and which features of the organotypic brain slice culture promoted microglia ramification. the potential of cells derived from embryonic yolk sac, embryonic aorta-gonad-mesonephros and adult blood monocytes was examined. these tissues were co-cultured with brain s...
متن کاملOrganotypic hippocampal slice cultures.
The hippocampus, a component of the limbic system, plays important roles in long-term memory and spatial navigation. Hippocampal neurons can modify the strength of their connections after brief periods of strong activation. This phenomenon, known as long-term potentiation (LTP) can last for hours or days and has become the best candidate mechanism for learning and memory. In addition, the well ...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2009
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2008.11.016